View source for Tests:Mycobacteria Tuberculosis, Sputum

{{DISPLAYTITLE: Mycobacterium tuberculosis (TB) PCR, Sputum}}
 
[[Category:Tests]]

[[Category:Microbiology]] 

{| class="wikitable"
! colspan="2" |
== Overview ==
|-
|Department
|Microbiology -  Molecular
|-
|Test Code
|TB
|-
|Synonyms
|Mycobacterium tuberculosis, 
TB
MTB
ZN
AFB
|-
|Availability 
|Monday to Friday
Not routinely available out of hours
|-
|Turnaround Time
|24 - 72 hours
|-
|Stability - Add test
|
|-
! colspan="2" |
== Specimen  Requirements ==
|-
|Specimen 
|Sputum
|-
|Preferred
|Only after consultation with the Clinical Microbiologist and/or Consultant Physician
|-
|Acceptable
|2 mL Sputum sample - induced or expectorated
|-
|Unacceptable 
|Leaking samples
Unlabelled samples
|-
|Specimen Transportation
|ASAP to Laboratory
|-
|Paediatric  Requirements 
|2 mL Sputum sample - induced or expectorated
|-
! colspan="2" |
== Aliquot  Requirements ==
|-
|Sample 
|
|-
|Preferred Volume
|
|-
|Minimum Volume
|
|-
|Aliquot Transportation
|If there is a delay in getting sample to the laboratory, please store sample chilled (2 - 8  degrees Celsius)
|-
! colspan="2" |
== Clinical  Information ==
|-
|Reference  Intervals
|Results reported  as MTB Complex DNA Result: DETECTED or NOT Detected.

If MTB Complex  DNA DETECTED - Rifampicin resistance will be reported as DETECTED or NOT  Detected.

These results  and susceptibilities will be confirmed upon TB Culture testing at reference  laboratory.
|-
|Test Method
|Semi-quantitative,  nested real-time PCR for the detection of Mycobacterium tuberculosis (MTB)  complex DNA.
|-
|Advanced Notice
|Not required
|-
|Patient Preparation
|Not Required
|-
! colspan="2" |
== Comments ==
|-
| colspan="2" |Sputum TB PCR requests can only be approved after consultation with Clinical Microbiologist and/or Consultant Physician.
The detection of the M. tuberculosis complex DNA and the detection of Rifampicin-resistance associated mutations of the rpoB gene from direct sputum.

All sputum samples requested for TB PCR will be forwarded to a reference laboratory for TB Culture (Taranaki Pathology Services testinfo-viewer (labapps.nz) )



''Please note:''  Culture remains the gold standard for the detection of M. tuberculosis.



TB PCR requests will be performed on sputum samples from patients suspected of having TB and/or where there are public health or infection control concerns. 

All requests should include relevant clinical details.

Specimens that fail for technical reasons will be forwarded to the reference laboratory for routine TB Culture. 

TB PCR may be re-tested dependant on volume of sample received, or if a re-collect is possible, meaning results will be delayed.

If sample volume does not meet requirements the requestor will be contacted, and the sample will be referred for routine TB Culture.
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