Tests:Mycobacteria Tuberculosis, Sputum: Difference between revisions

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Tests:Mycobacteria Tuberculosis, Sputum
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Created page with "{{DISPLAYTITLE: Mycobacteria Tuberculosis, Sputum}} Full Test List Microbiology Tests {| class="wikitable" ! colspan="2" | ===''Overview''=== |- |Department |Microbiology - Molecular |- |Test Code |TB |- |Synonyms |Mycobacteria tuberculosis, TB, MTB, ZN, AFB |- |Turnaround Time |24 - 72 hours |- ! colspan="2" | ===''Specimen Requirements''=== |- |Specimen Type |Sputum |- |Specimen Transportation |ASAP to Laboratory |- |Preferred | |- |Acceptable | |-..."
 
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{| class="wikitable"
{| class="wikitable"
! colspan="2" |
! colspan="2" |
===''Overview''===
== Overview ==
|-
|-
|Department
|Department
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|-
|-
|Synonyms
|Synonyms
|Mycobacteria tuberculosis, TB, MTB, ZN, AFB
|Mycobacteria tuberculosis,  
TB
MTB
ZN
AFB
|-
|Test Availability
|Monday to Friday
Not routinely available out of hours
|-
|-
|Turnaround Time
|Turnaround Time
|24 - 72 hours
|24 - 72 hours
|-
|Stability - Add test
|
|-
|-
! colspan="2" |
! colspan="2" |
===''Specimen  Requirements''===
== Specimen  Requirements ==
|-
|-
|Specimen Type
|Specimen Type
|Sputum
|Sputum
|-
|Specimen  Transportation
|ASAP to  Laboratory
|-
|-
|Preferred
|Preferred
|
|Only after consultation with the Clinical Microbiologist and/or Consultant Physician
|-
|-
|Acceptable
|Acceptable
|
|2 mL Sputum sample - induced or expectorated
|-
|Unacceptable
|Leaking samples
Unlabelled samples
|-
|Specimen Transportation
|ASAP to Laboratory
|-
|-
|Pediatric Requirements 
|Paediatric Requirements 
|
|2 mL Sputum sample - induced or expectorated
|-
|-
! colspan="2" |
! colspan="2" |
===''Aliquot  Requirements''===
== Aliquot  Requirements ==
|-
|-
|Sample Type
|Sample Type
|
|-
|Preferred Volume
|
|
|-
|-
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|-
|-
|Aliquot  Transportation
|Aliquot  Transportation
|If there is a delay in getting sample to the laboratory, please store sample chilled (2 - 8  degrees Celsius)
|If there is a delay in getting sample to the laboratory, please store sample chilled (2 - 8  degrees Celsius)
|-
|-
! colspan="2" |
! colspan="2" |
===''Clinical  Information''===
== Clinical  Information ==
|-
|-
|Reference  Intervals
|Reference  Intervals
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|-
|-
! colspan="2" |
! colspan="2" |
===''Comments''===
== Comments ==
|-
|-
| colspan="2" |*Only after  consultation with the Clinical Microbiologist and/or Consultant Physician*
| colspan="2" |Sputum TB PCR requests can only be approved after consultation with Clinical Microbiologist and/or Consultant Physician.
 
The detection of the M. tuberculosis complex DNA and the detection of Rifampicin-resistance associated mutations of the rpoB gene from direct sputum.
Sputum TB PCR requests can only be approved after consultation with Clinical Microbiologist and/or Consultant Physician.
 
 
The detection of the M. tuberculosis complex DNA and the detection of Rifampicin-resistance associated mutations of the rpoB gene from direct sputum.
 
 
All sputum  samples requested for TB PCR will be forwarded to a reference laboratory for  TB Culture (Taranaki Pathology Services testinfo-viewer (labapps.nz) )
 
 
Please note:  Culture remains the gold standard for the detection of M. tuberculosis.


All sputum samples requested for TB PCR will be forwarded to a reference laboratory for TB Culture (Taranaki Pathology Services testinfo-viewer (labapps.nz) )


TB PCR requests  will be performed on sputum samples from patients suspected of having TB  and/or where there are public health or infection control concerns.


''Please note:''  Culture remains the gold standard for the detection of M. tuberculosis.


All requests  should include relevant clinical details.


Specimens that  fail for technical reasons will be forwarded to the reference laboratory for  routine TB Culture.  
TB PCR requests will be performed on sputum samples from patients suspected of having TB and/or where there are public health or infection control concerns.  


All requests should include relevant clinical details.


TB PCR may be  re-tested dependant on volume of sample received, or if a re-collect is  possible, meaning results will be delayed.
Specimens that fail for technical reasons will be forwarded to the reference laboratory for routine TB Culture.  


TB PCR may be re-tested dependant on volume of sample received, or if a re-collect is possible, meaning results will be delayed.


If sample volume does not meet requirements the requestor will be contacted, and the sample will be referred for routine TB Culture.
If sample volume does not meet requirements the requestor will be contacted, and the sample will be referred for routine TB Culture.
|}
|}

Revision as of 15:48, 17 March 2025


Overview

Department Microbiology - Molecular
Test Code TB
Synonyms Mycobacteria tuberculosis,

TB MTB ZN AFB

Test Availability Monday to Friday

Not routinely available out of hours

Turnaround Time 24 - 72 hours
Stability - Add test

Specimen Requirements

Specimen Type Sputum
Preferred Only after consultation with the Clinical Microbiologist and/or Consultant Physician
Acceptable 2 mL Sputum sample - induced or expectorated
Unacceptable Leaking samples

Unlabelled samples

Specimen Transportation ASAP to Laboratory
Paediatric Requirements  2 mL Sputum sample - induced or expectorated

Aliquot Requirements

Sample Type
Preferred Volume
Minimum Volume
Aliquot Transportation If there is a delay in getting sample to the laboratory, please store sample chilled (2 - 8 degrees Celsius)

Clinical Information

Reference Intervals Results reported as MTB Complex DNA Result: DETECTED or NOT Detected.

If MTB Complex DNA DETECTED - Rifampicin resistance will be reported as DETECTED or NOT Detected.

These results and susceptibilities will be confirmed upon TB Culture testing at reference laboratory.

Test Method Semi-quantitative, nested real-time PCR for the detection of Mycobacterium tuberculosis (MTB) complex DNA.
Advanced Notice Not required
Patient Preparation Not Required

Comments

Sputum TB PCR requests can only be approved after consultation with Clinical Microbiologist and/or Consultant Physician.

The detection of the M. tuberculosis complex DNA and the detection of Rifampicin-resistance associated mutations of the rpoB gene from direct sputum.

All sputum samples requested for TB PCR will be forwarded to a reference laboratory for TB Culture (Taranaki Pathology Services testinfo-viewer (labapps.nz) )


Please note: Culture remains the gold standard for the detection of M. tuberculosis.


TB PCR requests will be performed on sputum samples from patients suspected of having TB and/or where there are public health or infection control concerns.

All requests should include relevant clinical details.

Specimens that fail for technical reasons will be forwarded to the reference laboratory for routine TB Culture.

TB PCR may be re-tested dependant on volume of sample received, or if a re-collect is possible, meaning results will be delayed.

If sample volume does not meet requirements the requestor will be contacted, and the sample will be referred for routine TB Culture.

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