Tests:Iron Studies, Serum: Difference between revisions

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Tests:Iron Studies, Serum
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{| class="wikitable"
{| class="wikitable"
! colspan="2" |
! colspan="2" |
===''Overview''===
== Overview ==
|-
|-
|Department
|Department
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|Test Code
|Test Code
|PIS
|PIS
|-
|Constituent Tests
|Iron
Transferrin
% Saturation
Ferritin
|-
|-
|Synonyms  
|Synonyms  
|TIBC (Superseded by Iron Studies)  
|TIBC (superseded by Iron Studies)


Iron Saturation   
Iron Saturation   
|-
|Availability
|Monday - Sunday, testing routinely available out of hours
|-
|-
|Turnaround Time
|Turnaround Time
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|-
|-
|Stability – Add test
|Stability – Add test
|
|Full Iron Studies - 8 hours
Ferritin - 5 days
|-
|-
! colspan="2" |
! colspan="2" |
===''Specimen Requirements''===
== Specimen Requirements ==
|-
|Specimen Type
|
|-
|-
|Specimen Transportation
|Specimen
|
|Plasma or Serum
|-
|-
|Preferred  
|Preferred  
|6 mL Plain <span style="background:#e30022"> Red-Top</span>
|LiHep <span style="background:#3cb371"> Green-Top </span> tube
|-
|-
|Acceptable  
|Acceptable  
|4 mL LiHep <span style="background:#3cb371"> Green-Top </span>
|<span style="background:#e30022"> Red-Top</span> or Yellow-Top tube
|-
|Unacceptable
|Grossly haemolysed
''Moderately haemolysed samples will only have Ferritin reported''
|-
|Specimen Transportation
|
|-
|-
|Pediatric Requirements
|Paediatric Requirements
| 600 μL <span style="background:#99e6b3"> Li-Hep Microtainer </span>
| 600 μL <span style="background:#99e6b3"> Li-Hep Microtainer </span>
|-
|-
! colspan="2" |
! colspan="2" |
===''Aliquot Requirements''===
== Aliquot Requirements ==
|-
|Sample
|Serum or Plasma
|-
|-
|Sample Type
|Preferred Volume
|
|0.5 mL
|-
|-
| Minimum Volume
| Minimum Volume
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|-
|-
! colspan="2" |
! colspan="2" |
===''Clinical Information''===
== Clinical Information ==
|-
|-
|Reference Intervals
|Reference Intervals
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|>18 +  Adult    
|>18 +  Adult    
|10 – 30
|10 – 30
|-
| colspan="2" |
|-
|-
|'''''Transferrin      '''''
|'''''Transferrin      '''''
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|>3 years +  Adult  
|>3 years +  Adult  
| 2.0 – 3.5
| 2.0 – 3.5
|-
| colspan="2" |
|-
|-
|'''''% Saturation'''''
|'''''% Saturation'''''
| 16-45%  (transferrin saturation)
| 16-45%  (transferrin saturation)
|-
| colspan="2" |
|-
|-
|'''''Ferritin'''''
|'''''Ferritin'''''
|'''''Reference Interval'''''
|'''''µ''g/L'''
|-
|-
|0 – 15 years
|0 – 15 years
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|-
|-
|Test Method
|Test Method
|Roche Cobas 6000 analyser using Roche Reagent.   
|Iron and Transferrin:  Abbott Alinity c series analyser using Abbott reagents.
 
Iron:                Ferene colourimetric assay
 
Transferrin:  Immunoturbidimetric assay
 
 
Ferritin:
Abbott Alinity i series analyser using Abbott reagents.
 
Chemiluminescent microparticle immunoassay (CMIA)
|-
|-
|Advanced Notice
|Advanced Notice
|Not Required  
|Not required  
|-
|-
| Patient Preparation
| Patient Preparation
|Not Required  
|Not required  
|-
|-
! colspan="2" |
! colspan="2" |
===''Comments''===
== Comments ==
|-
|-
| colspan="2" |Includes Iron, Transferrin, Ferritin and % saturation
| colspan="2" |
|}
|}
{{DocMeta
| owner = Crossley1
| review_ping =
}}

Latest revision as of 11:42, 30 January 2026

Overview

Department Biochemistry
Test Code PIS
Constituent Tests Iron

Transferrin

% Saturation

Ferritin

Synonyms TIBC (superseded by Iron Studies)

Iron Saturation 

Availability Monday - Sunday, testing routinely available out of hours
Turnaround Time 2 – 6 hrs
Stability – Add test Full Iron Studies - 8 hours

Ferritin - 5 days

Specimen Requirements

Specimen Plasma or Serum
Preferred LiHep Green-Top tube
Acceptable Red-Top or Yellow-Top tube
Unacceptable Grossly haemolysed

Moderately haemolysed samples will only have Ferritin reported

Specimen Transportation
Paediatric Requirements 600 μL Li-Hep Microtainer

Aliquot Requirements

Sample Serum or Plasma
Preferred Volume 0.5 mL
Minimum Volume
Aliquot Transportation

Clinical Information

Reference Intervals
Iron   µmol/L
0 – 2 months             10 – 31
>2 months - 1 year       4 – 27
>1 - 3 years       5 – 23
>3 - 10 years         6 – 25
>10 - 18 years       8 – 32
>18 + Adult     10 – 30
Transferrin       g/L
0-2 months 1.3 – 2.3
> 2 months - 3 years     1.6 – 3.3
>3 years + Adult   2.0 – 3.5
% Saturation 16-45% (transferrin saturation)
Ferritin µg/L
0 – 15 years 15 – 150
Female
15 – 50 years> 20 – 200
> 50 years 20 – 350
Male
>15 – 30 years 20 – 350
> 30 years 20 – 500
Test Method Iron and Transferrin: Abbott Alinity c series analyser using Abbott reagents.

Iron: Ferene colourimetric assay

Transferrin: Immunoturbidimetric assay


Ferritin: Abbott Alinity i series analyser using Abbott reagents.

Chemiluminescent microparticle immunoassay (CMIA)

Advanced Notice Not required  
Patient Preparation Not required  

Comments

Owner Clair Crossley (Crossley1)
Last reviewed 30.01.2026
Next review 30.01.2027 (OK)
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