Tests:Influenza A B: Difference between revisions

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Tests:Influenza A B
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{{DISPLAYTITLE: Influenza A B - RSV}}  
{{DISPLAYTITLE: Influenza A, B and RSV}}  


[[Category:Tests]]
[[Category:Tests]]


[[Category:Microbiology]]  
[[Category:Microbiology]]  
[[Category:Hawera]]


{| class="wikitable"
{| class="wikitable"
! colspan="2" |
! colspan="2" |
=== ''Overview'' ===
== Overview ==
|-
|-
|Department
|Department
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Respiratory Syncytial Virus
Respiratory Syncytial Virus
|-
|Base Lab Availability
|Monday - Sunday, testing routinely available out of hours
|-
|Hawera Lab Availability
|Monday - Friday, 8am to 4 30pm
|-
|-
|Turnaround Time
|Turnaround Time
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|-
|-
|Stability – Add test
|Stability – Add test
|
|Add-ons can be made if an appropriate sample has already been received for other PCR testing within one week of specimen collection.
|-
|-
! colspan="2" |
! colspan="2" |
=== ''Specimen Requirements'' ===
== Specimen Requirements ==
|-
|-
|Specimen Type
|Specimen  
|Nasopharyngeal swab in VTM (Viral transport media) 
|Swab
|-
|Specimen Transportation
|ASAP to Laboratory 
|-
|-
|Preferred
|Preferred
|
|Nasopharyngeal swab in VTM (Viral transport media) 
Samples should be taken as early as possible within the first 3-5 days after onset of symptoms.
|-
|-
|Acceptable
|Acceptable
|
|
|-
|-
|Pediatric Requirements   
|Unacceptable
|Leaking samples
Unlabelled and mislabelled samples
|-
|Specimen Transportation
|ASAP to Laboratory 
|-
|Paediatric Requirements   
|Paediatric nasopharyngeal swab in VTM (Viral transport  media)   
|Paediatric nasopharyngeal swab in VTM (Viral transport  media)   
|-
|-
! colspan="2" |
! colspan="2" |
=== ''Aliquot Requirements'' ===
== Aliquot Requirements ==
|-
|-
|Sample Type
|Sample  
|
|-
|Preferred Volume
|
|
|-
|-
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|-
|-
! colspan="2" |
! colspan="2" |
=== ''Clinical Information'' ===
== Clinical Information ==
|-
|-
|Reference Intervals
|Reference Intervals
|Results reported as DETECTED or NOT Detected for Influenza A, Influenza B and RSV.
|Results reported as DETECTED or NOT Detected for Influenza A, Influenza B and RSV.  
 
 
Specimens that fail for technical reasons will be  tested by another method and/or reflex tested for the full respiratory  multiplex panel, meaning results will be delayed.  


Specimens that fail for technical reasons will be tested by another method and/or reflex tested for the full respiratory multiplex panel, meaning results will be delayed.


Single or multiple viruses may be detected.
Single or multiple viruses may be detected.  
|-
|-
|Test Method
|Test Method
|Real-time RT-PCR test on the GeneXpert analyser for the detection of Influenza A (Flu A), Influenza B (Flu B), and Respiratory Syncytial Virus (RSV).  
|Real-time RT-PCR test on the GeneXpert analyser for the detection of Influenza A (Flu A), Influenza B (Flu B), and Respiratory Syncytial Virus (RSV).  


Note if a “QUAD” swab is requested then Influenza A + B, RSV, and SARS-CoV-2 are tested.
Note if a “QUAD” swab is requested then Influenza A + B, RSV, and SARS-CoV-2 are tested.
|-
|-
|Advanced Notice
|Advanced Notice
|Samples should be taken as early as possible within the  first 3-5 days after onset of symptoms. 
|
|-
|-
|Patient Preparation
|Patient Preparation
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|-
|-
! colspan="2" |
! colspan="2" |
=== ''Comments'' ===
== Comments ==
|-
|-
| colspan="2" |For mainstream routine respiratory testing during Influenza/RSV season.  
| colspan="2" |For mainstream routine respiratory testing during Influenza/RSV season.  


Rapid screening using molecular PCR to identify key respiratory viruses during outbreaks.  
Rapid screening using molecular PCR to identify key respiratory viruses during outbreaks.  


In cases that clinically justify a wider selection of respiratory pathogens, reflex testing can be performed at a reference laboratory using our Respiratory multiplex assay by contacting the laboratory directly on extn 7878 or by stating clearly on original request: "FULL RESPIRATORY MULTIPLEX" with clear clinical details supporting this request.   
In cases that clinically justify a wider selection of respiratory pathogens, reflex testing can be performed at a reference laboratory using our Respiratory multiplex assay by contacting the laboratory directly on extn 7878 or by stating clearly on original request: "FULL RESPIRATORY MULTIPLEX" with clear clinical details supporting this request.   


SARS-CoV-2  (COVID-19), Full Respiratory Multiplex and Bordetella pertussis PCR may be carried out on the same nasopharyngeal swab.


SARS-CoV-2  (COVID-19), Full Respiratory Multiplex and Bordetella pertussis PCR may be  carried out on the same nasopharyngeal swab.
''Please note'': Testing of the full respiratory multiplex assay does not have the same turnaround time as Influenza A/B, RSV testing as the sample is forwarded to a reference laboratory.   
 
 
Please note: Testing of the full respiratory multiplex assay does not have the same turnaround time as Influenza A/B, RSV testing as the sample is forwarded to a reference laboratory.   
|}
|}
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Latest revision as of 07:39, 24 December 2025


Overview

Department Microbiology – Molecular
Test Code LABR
Synonyms Influenza (A or B)

Flu

RSV

Respiratory Syncytial Virus

Base Lab Availability Monday - Sunday, testing routinely available out of hours
Hawera Lab Availability Monday - Friday, 8am to 4 30pm
Turnaround Time 2 – 24 hours from receipt

Results may be delayed out of routine business hours.

Urgent requests will be prioritised

Stability – Add test Add-ons can be made if an appropriate sample has already been received for other PCR testing within one week of specimen collection.

Specimen Requirements

Specimen Swab
Preferred Nasopharyngeal swab in VTM (Viral transport media) 

Samples should be taken as early as possible within the first 3-5 days after onset of symptoms.

Acceptable
Unacceptable Leaking samples

Unlabelled and mislabelled samples

Specimen Transportation ASAP to Laboratory
Paediatric Requirements Paediatric nasopharyngeal swab in VTM (Viral transport media) 

Aliquot Requirements

Sample
Preferred Volume
Minimum Volume
Aliquot Transportation If there is a delay in getting sample to the laboratory, please store sample chilled (2 - 8 degrees Celsius)

Clinical Information

Reference Intervals Results reported as DETECTED or NOT Detected for Influenza A, Influenza B and RSV.

Specimens that fail for technical reasons will be tested by another method and/or reflex tested for the full respiratory multiplex panel, meaning results will be delayed.

Single or multiple viruses may be detected.

Test Method Real-time RT-PCR test on the GeneXpert analyser for the detection of Influenza A (Flu A), Influenza B (Flu B), and Respiratory Syncytial Virus (RSV).

Note if a “QUAD” swab is requested then Influenza A + B, RSV, and SARS-CoV-2 are tested.

Advanced Notice
Patient Preparation To minimise inhibition in the assay, please ensure the patients blow their nose prior to taking a nasopharyngeal swab

Comments

For mainstream routine respiratory testing during Influenza/RSV season.

Rapid screening using molecular PCR to identify key respiratory viruses during outbreaks.

In cases that clinically justify a wider selection of respiratory pathogens, reflex testing can be performed at a reference laboratory using our Respiratory multiplex assay by contacting the laboratory directly on extn 7878 or by stating clearly on original request: "FULL RESPIRATORY MULTIPLEX" with clear clinical details supporting this request.

SARS-CoV-2 (COVID-19), Full Respiratory Multiplex and Bordetella pertussis PCR may be carried out on the same nasopharyngeal swab.

Please note: Testing of the full respiratory multiplex assay does not have the same turnaround time as Influenza A/B, RSV testing as the sample is forwarded to a reference laboratory. 

Owner Melanie Williams (Williams1)
Last reviewed 24.12.2025
Next review 24.12.2026 (OK)
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