Tests:Bordetella Pertussis PCR: Difference between revisions
Created page with "{{DISPLAYTITLE: Bordetella Pertussis PCR}} Full Test List Microbiology Tests {| class="wikitable" ! colspan="2" | == Overview == |- |Department |Microbiology - Molecular |- |Test Code |BPCR |- |Synonyms |Bordetella pertussis PCR whooping cough PCR |- |Test Availabilty |Monday - Friday 8 am - 4 pm |- |Turnaround Time |72 hours |- |Stability - Add test | |- ! colspan="2" | == Specimen Requirements == |- |Specimen Type | |- |Specimen Transportation |ASAP t..." |
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== Clinical Information == | == Clinical Information == | ||
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|Diagnostic use/ Indications | |||
|Pertussis, also known as whopping cough, is a highly contagious respiratory illness caused by a Gram-negative coccobacillus ''Bordetella pertussis''. | |||
Pertussis is a Section B Notifiable Infectious Diseases Under the Health Act 1956. | |||
Nucleic acid detection after PCR amplification is more sensitive than culture. | |||
During the first 3 weeks of cough PCR sensitivity for detection of ''B. pertussis'' is high but drops substantially thereafter. | |||
For optimal assay sensitivity specimens should be taken from the posterior nasopharynx. | |||
Other species of the genus B. parapertussis and B. holmesii are known to exhibit clinical manifestations of respiratory infection similar to a pertussis-like disease, | |||
however these are not notifiable. | |||
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|Reference Intervals | |Reference Intervals | ||
Revision as of 13:56, 12 March 2025
Overview | |
|---|---|
| Department | Microbiology - Molecular |
| Test Code | BPCR |
| Synonyms | Bordetella pertussis PCR
whooping cough PCR |
| Test Availabilty | Monday - Friday
8 am - 4 pm |
| Turnaround Time | 72 hours |
| Stability - Add test | |
Specimen Requirements | |
| Specimen Type | |
| Specimen Transportation | ASAP to Laboratory
If there is a delay in getting sample to the laboratory, please store sample chilled (2 - 8 degrees Celsius) |
| Preferred | Nasopharyngeal, Pernasal swab in Universal / Virus transport medium (UTM/VTM) |
| Acceptable | |
| Unacceptable | Leaking samples
Unlabelled samples |
| Paediatric Requirements | Nasopharyngeal, Pernasal swab in UTM/VTM |
Aliquot Requirements | |
| Sample Type | |
| Preferred Volume | |
| Minimum Volume | |
| Aliquot Transportation | |
Clinical Information | |
| Diagnostic use/ Indications | Pertussis, also known as whopping cough, is a highly contagious respiratory illness caused by a Gram-negative coccobacillus Bordetella pertussis.
Pertussis is a Section B Notifiable Infectious Diseases Under the Health Act 1956. Nucleic acid detection after PCR amplification is more sensitive than culture. During the first 3 weeks of cough PCR sensitivity for detection of B. pertussis is high but drops substantially thereafter. For optimal assay sensitivity specimens should be taken from the posterior nasopharynx. Other species of the genus B. parapertussis and B. holmesii are known to exhibit clinical manifestations of respiratory infection similar to a pertussis-like disease, however these are not notifiable. |
| Reference Intervals | Results reported as DETECTED or NOT Detected for
|
| Test Method | Real-time PCR for the qualitative detection and differentiation of DNA from Bordetella pertussis, Bordetella parapertussis and/or Bordetella holmesii. |
| Advanced Notice | Not required |
| Patient Preparation | Not required |
Comments | |
| Influenza A/B and RSV, SAR-CoV-2 and Full Respiratory Multiplex may be carried out on the same nasopharyngeal swab.
Please note: Testing of the full respiratory multiplex assay does not carry the same turnaround times of the Influenza A/B, RSV and SARS-CoV-2 test(s) as the sample is forwarded to a reference laboratory. | |