Tests:Infectious Mononucleosis, Plasma: Difference between revisions
Tests:Infectious Mononucleosis, Plasma
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== | == Overview == | ||
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|Department | |Department | ||
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IM | IM | ||
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|Test Availability | |||
|Monday - Sunday, testing routinely available out of hours | |||
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|Turnaround Time | |Turnaround Time | ||
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! colspan="2" | | ! colspan="2" | | ||
== | == Specimen Requirements == | ||
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|Specimen Type | |Specimen Type | ||
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== | == Aliquot Requirements == | ||
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|Sample Type | |Sample Type | ||
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|Preferred Volume | |||
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== Clinical Information == | |||
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|Reference Intervals | |Reference Intervals | ||
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== | == Comments == | ||
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| colspan="2" |This test screens for the presence of heterophile antibodies. EBV serology is the preferred test in cases of suspected infectious mononucleosis due to the inferior sensitivity of the monospot test. | | colspan="2" |This test screens for the presence of heterophile antibodies. EBV serology is the preferred test in cases of suspected infectious mononucleosis due to the inferior sensitivity of the monospot test. | ||
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Limitations of the monospot test: | ''Limitations of the monospot test:'' | ||
During the first week of disease 30-40% of patients will produce a false negative result. Suggest repeat EBV serology if symptoms persist. | During the first week of disease 30-40% of patients will produce a false negative result. Suggest repeat EBV serology if symptoms persist. | ||
Revision as of 09:57, 17 March 2025
Overview | |
|---|---|
| Department | Haematology |
| Test Code | IM |
| Synonyms | Monospot
Paul Bunnell Glandular fever IM |
| Test Availability | Monday - Sunday, testing routinely available out of hours |
| Turnaround Time | 1-5 hrs |
| Stability – Add test | 24 hrs at room temp, 3 days at 4 degrees Celsius. |
Specimen Requirements | |
| Specimen Type | |
| Specimen Transportation | |
| Preferred | 6mL Plain Red-Top |
| Acceptable | 4mL EDTA Purple-Top , or Li-Hep Green-Top |
| Unacceptable | |
| Paediatric Requirements | 0.5 mL plain Red-Top, 500 μL EDTA Microtainer , or 600 μL Li-Hep Microtainer |
Aliquot Requirements | |
| Sample Type | |
| Preferred Volume | |
| Minimum Volume | |
| Aliquot Transportation | |
Clinical Information | |
| Reference Intervals | Positive test suggests the diagnosis of glandular fever in the context of appropriate symptoms and blood film changes such as reactive lymphocytosis. |
| Test Method | Clearview IM rapid immunoassay kit for detection of EBV IgM Heterophile Antibody. |
| Advanced Notice | |
| Patient Preparation | |
Comments | |
| This test screens for the presence of heterophile antibodies. EBV serology is the preferred test in cases of suspected infectious mononucleosis due to the inferior sensitivity of the monospot test.
The monospot test will not be performed on children <6yrs due to the poor sensitivity in this age group. Any sample with a negative monospot test result will be referred for EBV serology.
Limitations of the monospot test: During the first week of disease 30-40% of patients will produce a false negative result. Suggest repeat EBV serology if symptoms persist. 50% of children <6 years of age and 10-20% of adults will not produce heterophile IM antibodies. False positive results may be seen in cases of CMV, malaria, toxoplasmosis and some autoimmune diseases. | |