Tests:SARS-CoV-2: Difference between revisions

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Tests:SARS-CoV-2
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=== ''Overview'' ===
== Overview ==
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|Department
|Department
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Urgent requests will be prioritised
Urgent requests will be prioritised
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|Stability – Add test
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! colspan="2" |
=== ''Specimen Requirements'' ===
== Specimen Requirements ==
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|Specimen Type
|Specimen Type
|Nasopharyngeal swab in VTM (Viral transport  medium)   
|Swab
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|Preferred
|Nasopharyngeal swab in VTM (Viral transport  medium) 
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|Acceptable
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|Unacceptable
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|Specimen Transportation
|Specimen Transportation
|ASAP to Laboratory   
|ASAP to Laboratory   
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|Pediatric Requirements   
|Paediatric Requirements   
|Paediatric nasopharyngeal swab in VTM (Viral transport  medium)   
|Paediatric nasopharyngeal swab in VTM (Viral transport  medium)   
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! colspan="2" |
! colspan="2" |
=== ''Aliquot Requirements'' ===
== Aliquot Requirements ==
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|Sample Type
|Sample Type
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|Preferred Volume
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|
|-
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|Aliquot Transportation
|Aliquot Transportation
|If there is a delay in getting sample to the  laboratory, please store sample chilled (2 - 8 degrees Celsius)
|If there is a delay in getting sample to the  laboratory, please store sample chilled (2 - 8 degrees Celsius)
|-
|Stability – Add test
|
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! colspan="2" |
=== ''Clinical Information'' ===
== Clinical Information ==
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|Reference Intervals
|Reference Intervals
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=== ''Comments'' ===
== Comments ==
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| colspan="2" |Influenza A/B and RSV, Full Respiratory Multiplex and Bordetella pertussis PCR may be carried out on the same nasopharyngeal swab.  
| colspan="2" |Influenza A/B and RSV, Full Respiratory Multiplex and Bordetella pertussis PCR may be carried out on the same nasopharyngeal swab.  
 


Please note: Testing of the full respiratory multiplex assay does not have the same turnaround time as the Influenza A/B, RSV test as the sample is forwarded to a reference laboratory.   
''Please note:'' Testing of the full respiratory multiplex assay does not have the same turnaround time as the Influenza A/B, RSV test as the sample is forwarded to a reference laboratory.   
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Revision as of 15:26, 17 March 2025


Overview

Department Microbiology – Molecular
Test Code TCOV
Synonyms COVID-19

SARS-CoV-2

Novel Coronavirus

2019-nCov 

Turnaround Time 2 – 24 hours from receipt 

Results may be delayed out of routine business hours.

Urgent requests will be prioritised

Stability – Add test

Specimen Requirements

Specimen Type Swab
Preferred Nasopharyngeal swab in VTM (Viral transport medium) 
Acceptable
Unacceptable
Specimen Transportation ASAP to Laboratory
Paediatric Requirements Paediatric nasopharyngeal swab in VTM (Viral transport medium) 

Aliquot Requirements

Sample Type
Preferred Volume
Minimum Volume
Aliquot Transportation If there is a delay in getting sample to the laboratory, please store sample chilled (2 - 8 degrees Celsius)

Clinical Information

Reference Intervals Results will be reported as Novel Coronavirus RNA PCR DETECTED or NOT Detected. 

Ct values are available on request.

Specimens that fail for technical reasons will be tested by another platform or a repeat sample requested, this will mean a delay in results

Test Method Real-time RT-PCR test on the GeneXpert analyser for the qualitative detection of nucleic acid from the SARS-CoV-2 virus.

Note if a “QUAD” swab is requested then Influenza A + B, RSV, and SARS-CoV-2 are tested.

Advanced Notice Not required 
Patient Preparation To minimise inhibition in the assay, please ensure the patients blow their nose prior to taking a nasopharyngeal swab. 

Comments

Influenza A/B and RSV, Full Respiratory Multiplex and Bordetella pertussis PCR may be carried out on the same nasopharyngeal swab.

Please note: Testing of the full respiratory multiplex assay does not have the same turnaround time as the Influenza A/B, RSV test as the sample is forwarded to a reference laboratory.